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pbluescript (pbs) ii sk  (Agilent technologies)


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    Structured Review

    Agilent technologies pbluescript (pbs) ii sk
    Pbluescript (Pbs) Ii Sk, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbs+ii+sk/us11732247-282-25-31
    Average 90 stars, based on 1 article reviews
    pbluescript (pbs) ii sk - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Overexpression of transcription factor AP-2alpha suppresses mammary gland growth and morphogenesis.
    Article Snippet: AP-2 transcription factors are key regulators of mouse embryonic development.. Aberrant expression of these genes has also been linked to the progression of human breast cancer.. Here, we have investigated the role of the AP-2 gene family in the postnatal maturation of the mouse mammary gland.

    Article Title: Fatty Acid Competition as a Mechanism by Which Enterobacter cloacae Suppresses Pythium ultimum Sporangium Germination and Damping-Off
    Article Snippet: pUC19 , Cloning vector, Amp r , 58. .. pBS II SK + , Cloning vector, Amp r , Stratagene. .. PUTKm , Tn 5 -based delivery plasmid, Km r Amp r , 20.

    Clone Assay:

    Article Title: Factors acting on Mos1 transposition efficiency.
    Article Snippet: .. Double-stranded oligonucleotides containing the 5' and 3' ITR (Table 3, 5'ITRup+5'ITRdw and 3'ITRup+3'ITRdw) were cloned in the SmaI restriction site of pBS II SK+ (Stratagene), giving the p5' and p3' constructs. ..

    Article Title: Factors acting on Mos1 transposition efficiency
    Article Snippet: .. A double-stranded 3'ITR oligonucleotide with Bam HI extremities was cloned at the Bam HI site of pBS II SK+ (Stratagene), to produce pBS-3'ITR. ..

    Article Title: Factors acting on Mos1 transposition efficiency.
    Article Snippet: .. A double-stranded 3'ITR oligonucleotide with BamHI extremities was cloned at the BamHI site of pBS II SK+ (Stratagene), to produce pBS-3'ITR. ..

    Article Title: Convergence of RNA cis Elements and Cellular Polyadenylation Factors in the Regulation of Human Cytomegalovirus UL37 Exon 1 Unspliced RNA Production
    Article Snippet: .. For in vitro transcription, the Target 1 unspliced minigene and UL37x1-UL37x2 spliced cDNA (p251 [ 46 ]) were PCR amplified and cloned into pBS II SK+ (Stratagene) generating p939 (unspliced), 1028 (unspliced), and p917 (spliced). ..

    Article Title: Factors acting on Mos1 transposition efficiency
    Article Snippet: .. Double-stranded oligonucleotides containing the 5' and 3' ITR (Table , 5'ITRup+5'ITRdw and 3'ITRup+3'ITRdw) were cloned in the Sma I restriction site of pBS II SK+ (Stratagene), giving the p5' and p3' constructs. ..

    Construct:

    Article Title: Factors acting on Mos1 transposition efficiency.
    Article Snippet: .. Double-stranded oligonucleotides containing the 5' and 3' ITR (Table 3, 5'ITRup+5'ITRdw and 3'ITRup+3'ITRdw) were cloned in the SmaI restriction site of pBS II SK+ (Stratagene), giving the p5' and p3' constructs. ..

    Article Title: Factors acting on Mos1 transposition efficiency
    Article Snippet: .. Double-stranded oligonucleotides containing the 5' and 3' ITR (Table , 5'ITRup+5'ITRdw and 3'ITRup+3'ITRdw) were cloned in the Sma I restriction site of pBS II SK+ (Stratagene), giving the p5' and p3' constructs. ..

    Derivative Assay:

    Article Title: A Rhizobium leguminosarum AcpXL Mutant Produces Lipopolysaccharide Lacking 27-Hydroxyoctacosanoic Acid
    Article Snippet: .. pBS II SK+ , 2.96-kb phagemid derived from pUC19, lacZ Amp r , Stratagene Corp.. ..

    Cloning:

    Article Title: Fatty Acid Competition as a Mechanism by Which Enterobacter cloacae Suppresses Pythium ultimum Sporangium Germination and Damping-Off
    Article Snippet: pUC19 , Cloning vector, Amp r , 58. .. pBS II SK + , Cloning vector, Amp r , Stratagene. .. PUTKm , Tn 5 -based delivery plasmid, Km r Amp r , 20.

    In Vitro:

    Article Title: Convergence of RNA cis Elements and Cellular Polyadenylation Factors in the Regulation of Human Cytomegalovirus UL37 Exon 1 Unspliced RNA Production
    Article Snippet: .. For in vitro transcription, the Target 1 unspliced minigene and UL37x1-UL37x2 spliced cDNA (p251 [ 46 ]) were PCR amplified and cloned into pBS II SK+ (Stratagene) generating p939 (unspliced), 1028 (unspliced), and p917 (spliced). ..

    Polymerase Chain Reaction:

    Article Title: Convergence of RNA cis Elements and Cellular Polyadenylation Factors in the Regulation of Human Cytomegalovirus UL37 Exon 1 Unspliced RNA Production
    Article Snippet: .. For in vitro transcription, the Target 1 unspliced minigene and UL37x1-UL37x2 spliced cDNA (p251 [ 46 ]) were PCR amplified and cloned into pBS II SK+ (Stratagene) generating p939 (unspliced), 1028 (unspliced), and p917 (spliced). ..

    Amplification:

    Article Title: Convergence of RNA cis Elements and Cellular Polyadenylation Factors in the Regulation of Human Cytomegalovirus UL37 Exon 1 Unspliced RNA Production
    Article Snippet: .. For in vitro transcription, the Target 1 unspliced minigene and UL37x1-UL37x2 spliced cDNA (p251 [ 46 ]) were PCR amplified and cloned into pBS II SK+ (Stratagene) generating p939 (unspliced), 1028 (unspliced), and p917 (spliced). ..



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    (A) A <t>pBluescript</t> II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.
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    pbluescript (pbs) ii sk  (Agilent technologies)
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    (A) A <t>pBluescript</t> II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.
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    pbluescript ii sk control plasmid  (Addgene inc)
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    (A) A <t>pBluescript</t> II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.
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    pbs ii sk  (Agilent technologies)
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    (A) A <t>pBluescript</t> II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.
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    Image Search Results


    (A) A pBluescript II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.

    Journal: PLOS ONE

    Article Title: DAPE cloning with modified primers for producing designated lengths of 3’ single-stranded ends in PCR products

    doi: 10.1371/journal.pone.0318015

    Figure Lengend Snippet: (A) A pBluescript II SK+ vector was chosen as a PCR template for amplifying the indicated lengths of fragments. Both PCR products, depicted in blue and red, were combined and assembled into the intact pBluescript II SK+ vector using SLIC techniques. The black lines represent the 15 bp complementary sequences of each product. (B) A schematic diagram of phosphodiester and phosphorothioate (PT) bonds used in the preparation of primer synthesis. (C) Primers for amplifying the two different regions of pBluescript II SK+ in (A) were modified with a 5’ end phosphate (P-15 mer-15 mer), a 5’ end phosphate together with five PTs (P-15 mer-5PT-10 mer), five PTs only (15 mer-5PT-10 mer), or were not modified (15 mer-15 mer). The orange braces represent the complementary sequences. (D) Transformation efficiencies were calculated by counting the number of colonies in triplicate experiments. 1×10 8 competent cells were used for transforming the mixture of 50 ng of each purified PCR product after undergoing SLIC experiments. Single asterisk; P-values lower than 0.05, double asterisk; lower than 0.01, triple asterisks; P-value lower than 0.001. All experiments were repeated three times. (E) Representative plates of (D) are shown. CFU; colony forming unit, Ctl; control, Lambda Exo; lambda exonuclease, T5 exo; T5 exonuclease.

    Article Snippet: 10 ng of pBluescript II SK+ was used as the PCR template. pX330-puro was a gift from Sandra Martha Gomes Dias (Addgene plasmid # 110403; http://n2t.net/addgene:110403 ; RRID:Addgene 110403).

    Techniques: Plasmid Preparation, Modification, Transformation Assay, Purification, Control